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To define new virtual input axes in Unity, you configure the key mapping in the Input Manager. The Input Manager is a specialized component that allows developers to set up and manage input settings for their game, including defining new axes for various types of input such as keyboard, mouse, and game controllers.

This component provides a user-friendly interface where developers can specify the names of the axes, the corresponding keys or buttons that trigger those axes, and additional properties such as sensitivity and gravity. By configuring these settings within the Input Manager, developers ensure that the game can accurately detect and respond to player input.

The other options do not serve this specific function. Player Settings focuses more on global settings of the game, such as resolution and graphics qualities. Action Manager typically relates to gameplay mechanics and does not directly deal with input axes. The Input Interface is a more general term that may refer to other aspects of input handling but is not the correct component for defining new virtual input axes. Thus, the Input Manager is the essential tool for setting up and customizing input controls in Unity.