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Unity does not support the MIDI audio format, which is primarily a protocol for communicating musical performance information rather than a format for storing audio samples. Unlike WAV, MP3, and OGG formats that contain actual audio data and can be directly played back within Unity, MIDI files are used for sequencing music and require additional software or hardware to interpret the MIDI data into audible sound.

The other formats—WAV, MP3, and OGG—are widely used for audio playback in Unity projects. WAV files contain uncompressed audio and are often used for high-quality sounds. MP3 files provide a compressed format that reduces file size while maintaining reasonable audio quality, making them suitable for music and sound effects. OGG files, which use the Ogg Vorbis codec, offer another way to store compressed audio, often resulting in better quality at lower bit rates compared to MP3. Thus, these formats are fully supported and commonly utilized in game development with Unity.